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Western blot analysis of alpha Tubulin expression in Saccharomyces cerevisiae (yeast) cell lysate.

alpha Tubulin(BM3885)(MW:55KD)大鼠腦，大鼠腦，小鼠腦，小鼠腦組織，人Hela細胞裂解，免疫印跡分析。

Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody.

Figure 1. Western blot analysis of anti- alpha Tubulin antibody (BM1452). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SH-SY5Y whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse thymus tissue lysates.
Use mouse anti- alpha Tubulin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for alpha Tubulin at approximately 55KD. The expected band size for alpha Tubulin is at 50KD.

Figure 2. IHC analysis using anti- alpha Tubulin antibody (BM1452). detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of anti- ATP1A1 antibody (PB0504).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human U87-MG whole cell lysates,Lane 3: human HEK293 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human HEPG2 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: monkey COS-7 whole cell lysates,Lane 8: human K562 whole cell lysates.Use rabbit anti- ATP1A1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP1A1 at approximately 100-113KD. The expected band size for ATP1A1 at 113KD.

Western blot analysis of Sodium Potassium ATPase expression in HeLa cell lysate.

Western blot analysis of BCL2A1 expression in Raji cell lysate.

Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 2. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebra fish tissue lysates,
Lane 2: chicken heart tissue lysates,
Lane 3: chicken liver tissue lysates,
Lane 4: chicken brain tissue lysates,
Lane 5: monkey heart tissue lysates,
Lane 6: monkey lung tissue lysates,
Lane 7: monkey kidney tissue lysates,
Lane 8: monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 1. Western blot analysis of anti- β-Actin antibody (BM3873).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat heart tissue lysates,Lane 6: Rat brain tissue lysates,Lane 7: Rat PC-12 whole cell lysates,Lane 8: Mouse heart tissue lysates,Lane 9: Mouse brain tissue lysates,Lane 10: Mouse ANA-1 whole cell lysates.Use rabbit anti- β-Actin 1:3000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.

Figure 1. Western blot analysis of anti- β-Actin antibody (BM0627).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat brain tissue lysates,Lane 6: Rat PC-12 whole cell lysates,Lane 7: Mouse brain tissue lysates,Lane 8: Mouse ANA-1 whole cell lysates,Lane 9: Rabbit intestines tissue lysates,Lane 10: Rabbit intestines tissue lysates,Lane 11: Pig intestines tissue lysates.Use rabbit anti- β-Actin 1:5000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.

Figure 2.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Mouse Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 3.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Rat Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 4.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Intestinal Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 5.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Lung Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 6.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.

Western blot analysis of beta Actin expression in (1) Hela cell lysate, (2) Human liver lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) PC12 cell lysate, (6) Rat heart lysate.

Western blot analysis of beta III Tubulin expression in (1) HeLa cell lysate; (2) PC-12 cell lysate; (3) Mouse brain lysate; (4) Rat brain lysate with beta Tubulin III Antibody.

Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate.

Figure 1. Western blot analysis of anti- TUBB antibody (M01857-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human HELA whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human HL-60 whole cell lysates,Lane 5: human Raji whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates.Use rabbit anti- TUBB 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for TUBB at approximately 55KD. The expected band size for TUBB is at 55KD.

Figure 2.ICC analysis using anti- TUBB antibody (M01857-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).

Figure 3. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

Figure 1. Western blot analysis of anti- Beta III Tubulin antibody (A01857-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: SH-SY5Y whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: C6 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: 3T3-L1 whole cell lysates.
Use rabbit anti- Beta III Tubulin 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Beta III Tubulin at approximately 54KD. The expected band size for Beta III Tubulin is at 50KD.

Figure 2. IHC analysis of Beta III Tubulin using anti-Beta III Tubulin antibody (A01857-1).Beta III Tubulin was detected in paraffin-embedded section of human glioma tissues. anti-Beta III Tubulin Antibody (A01857-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Beta III Tubulin using anti-Beta III Tubulin antibody (A01857-1).Beta III Tubulin was detected in paraffin-embedded section of human mammary cancer tissues. anti-Beta III Tubulin Antibody (A01857-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 tissue lysates,Lane 2: monkey COS-7 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Hela whole cell lysates,Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.

Figure 2. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat liver whole cell lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain whole cell lysates,Lane 5: mouse NIH3T3, whole cell lysates,Lane 6: mouse RAW264.7 whole cell lysates.Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.

Figure 3. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

Figure 4. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

Figure 5.Flow cytometry analysis of U937 cell(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight488. Unlabelled sample (Red line).

Figure 1.IHC analysis using anti-BrdU antibody (BM0201). detected in paraffin-embedded section of HELA cells. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 5. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

Figure 1. Western blot analysis of anti- COX4I1 antibody (M05442-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human T47D whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates.
Use mouse anti- COX4I1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for COX4I1 at approximately 17KD. The expected band size for COX4I1 is at 20KD.

Figure 2. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human colon cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

Figure 3. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

Figure 4. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

Figure 1. Western blot analysis of COX IV using anti-COX IV antibody (A05442-1). Lane 1: human HepG2 whole cell lysates,Lane 2: human SGC-7901 whole cell lysates. anti-COX IV antigen affinity purified polyclonal antibody (Catalog # A05442-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for COX IV at approximately 17-20KD. The expected band size for COX IV is at 20KD.

Figure 2. Western blot analysis of COX IV using anti-COX IV antibody (A05442-1). Lane 1: rat heart tissue lysates,Lane 2: mouse heart tissue lysates. anti-COX IV antigen affinity purified polyclonal antibody (Catalog # A05442-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for COX IV at approximately 17-20KD. The expected band size for COX IV is at 20KD.

Figure 3. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse small intestine tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 4. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat liver tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 5. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat lung tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 6. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 7. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 8. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human placenta tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 9. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 10. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse kidney tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 11. ICC analysis of anti-COX4I1 antibody (A05442-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).

Figure 12. Flow cytometry analysis of U937 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

Western blot analysis of COX IV expression in HepG2 cell lysate.